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CALML3 ELISA Kit (Human) (GWB-KBBXA2)

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GWB-KBBXA2

96 Wells
$709.00
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Additional Information

Name CALML3 ELISA Kit (Human) (GWB-KBBXA2)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CALML3
Gene id 810
Alias Symbols Calmodulin-like protein 3, Calmodulin-related protein NB-1, CaM-like protein, CLP
Swissprot ID P27482
Protein Name Calmodulin-like protein 3
Kit Principle Genway Biotech CALML3 ELISA Kit (Human) (GWB-KBBXA2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALML3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CALML3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALML3 captured in the well.
Kit Component
ComponentAmount
Anti-CALML3 Microplate96 Wells (12 x 8 Well Strips)
CALML3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CALML3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_005176.1
Nucleotide Accession Num NM_005185.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXA2
Species Reactivity Human