Canine Alpha 1-Acid Glycoprotein (AGP) ELISA (GWB-374Z17)

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GWB-374Z17

96 Wells
$549.00
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Additional Information

Name Canine Alpha 1-Acid Glycoprotein (AGP) ELISA (GWB-374Z17)
Related Product Names Orosomucoid, Orosomucoid-1, OMD-1, AGP-1, AGP, Alpha-1-acid glycoprotein, Dog Orosomucoid ELISA Kit, Dog Alpha 1-Acid Glycoprotein ELISA, Dog AGP ELISA
Description of Target Functions as transport protein in the blood stream.
Kit Range 6.17 ng/ml - 1500 ng/ml
Sensitivity 1.706 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene id 100685620
Alias Symbols AGP1, ORM1, Alpha-1-acid glycoprotein 1
Swissprot ID F6Y713
Protein Name Alpha-1-acid glycoprotein
Kit Principle Genway Biotech Alpha 1-Acid Glycoprotein ELISA Kit (Dog) (GWB-374Z17) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-acid glycoprotein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-acid glycoprotein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-acid glycoprotein captured in the well.
Kit Component
ComponentAmount
Anti-Dog Alpha 1-Acid Glycoprotein Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Dog Alpha 1-Acid Glycoprotein Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Dog
Standard Range 1500 ng/ml-6.17 ng/ml
Product Format In Solution
Specimen/Volumes Refer To Manual
Concentration Lot Specific
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-AGP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-DOG AGPELISA MICRO PLATE
Anti-Dog AGP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. DOG AGP CALIBRATOR
The lyophilized Dog AGP calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Canine
Kit Size 96 well
Kit Duration 100 Minutes
Storage Refer to kit instructions for component storage
Datasheets/Manuals Printable datasheet for GWB-374Z17
Species Reactivity Dog
Intended Use Research Use Only
Additional Information Intended Use: The AGP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP in biological fluid of dogs.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Dog AGP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-DOG AGP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Dog AGP.
7. DOG AGP CALIBRATOR
Two vials containing Dog AGP calibrator.