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CANX ELISA Kit (Human) (GWB-KBBJN2)

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GWB-KBBJN2

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Additional Information

Name CANX ELISA Kit (Human) (GWB-KBBJN2)
Description of Target Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CANX
Gene id 821
Alias Symbols Calnexin, CNX, FLJ26570, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, P90
Swissprot ID P27824
Protein Name calnexin
Kit Principle Genway Biotech CANX ELISA Kit (Human) (GWB-KBBJN2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CANX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CANX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CANX captured in the well.
Kit Component
ComponentAmount
Anti-Glud1 Microplate96 Wells (12 x 8 Well Strips)
Glud1 Lyophilized Standard2 x 10 ng
100X Biotinylated Glud1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001019820
Nucleotide Accession Num NM_001024649
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJN2
Species Reactivity Human