Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the
Kit Range
0.156 - 10 ng/mL
Sensitivity
< 0.063 ng/mL
Sample Type
tissue homogenates, cell lysates and other biological fluids
Genway Biotech CANX ELISA Kit (Human) (GWB-KBBJN2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CANX has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CANX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CANX captured in the well.
