CAP1 ELISA Kit (Human) (GWB-KBBLA8) (Aviva Cat. No. OKCD00277)

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Please see item number OKCD00277 to view product details and for ordering information.


GWB-KBBLA8

Aviva Cat. No.: OKCD00277

Additional Information

Name CAP1 ELISA Kit (Human) (GWB-KBBLA8)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CAP1
Gene id 10487
Alias Symbols Adenylyl cyclase-associated protein 1, CAP, CAP 1, CAP1-PEN
Swissprot ID Q01518
Protein Name adenylyl cyclase-associated protein 1
Description of Target Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
Kit Principle Genway Biotech CAP1 ELISA Kit (Human) (GWB-KBBLA8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAP1 captured in the well.
Kit Component
ComponentAmount
Anti-LAMP1 Microplate96 Wells (12 x 8 Well Strips)
LAMP1 Lyophilized Standard2 x 10 ng
100X Biotinylated LAMP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001099000
Nucleotide Accession Num NM_001105530
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
84-98%87-103%89-98%97-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
89-103
Datasheets/Manuals Printable datasheet for GWB-KBBLA8
Species Reactivity Human