CAPNS1 ELISA Kit (Human) (GWB-KBBOY9)

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GWB-KBBOY9

96 Wells
$659.00
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Additional Information

Name CAPNS1 ELISA Kit (Human) (GWB-KBBOY9)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.126 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CAPNS1
Gene id 826
Alias Symbols 30K, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, CALPAIN4, Calpain regulatory subunit, Calpain small subunit 1, CANP, CANPS, CANP small subunit, CAPN4, CAPNS, CD
Swissprot ID P04632
Protein Name calpain small subunit 1
Description of Target Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Kit Principle Genway Biotech CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPNS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPNS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPNS1 captured in the well.
Kit Component
ComponentAmount
Anti-Crybb1 Microplate96 Wells (12 x 8 Well Strips)
Crybb1 Lyophilized Standard2 x 20 ng
100X Biotinylated Crybb1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001003962
Nucleotide Accession Num NM_001003962
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBOY9
Species Reactivity Human