| Name |
CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.126 ng/mL |
| Sample Type |
tissue homogenates and other biological fluids. |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CAPNS1 |
| Gene id |
826 |
| Alias Symbols |
30K, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calcium-dependent protease small subunit 1, CALPAIN4, Calpain regulatory subunit, Calpain small subunit 1, CANP, CANPS, CANP small subunit, CAPN4, CAPNS, CD |
| Swissprot ID |
P04632 |
| Protein Name |
calpain small subunit 1 |
| Description of Target |
Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. |
| Kit Principle |
Genway Biotech CAPNS1 ELISA Kit (Human) (GWB-KBBOY9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPNS1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPNS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPNS1 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-Crybb1 Microplate | 96 Wells (12 x 8 Well Strips) | | Crybb1 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated Crybb1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001003962 |
| Nucleotide Accession Num |
NM_001003962 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBOY9 |
| Species Reactivity |
Human |
