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96 Wells

Additional Information

Name CAPZb ELISA Kit (Human) (GWB-KBBRN7)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.052 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CAPZb
Gene id 832
Alias Symbols CAPB, CAPPB, CAPZ, CapZ beta, DKFZp686K0524, F-actin-capping protein subunit beta, FLJ12274, FLJ44693, MGC104401, MGC129749, MGC129750
Swissprot ID P47756
Protein Name F-actin-capping protein subunit beta
Description of Target This gene encodes the beta subunit of the barbed-end actin binding protein, which belongs to the F-actin capping protein family. The capping protein is a heterodimeric actin capping protein that blocks actin filament assembly and disassembly at the fast g
Kit Principle Genway Biotech CAPZb ELISA Kit (Human) (GWB-KBBRN7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAPZb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAPZb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAPZb captured in the well.
Kit Component
Anti-CAPZb Microplate96 Wells (12 x 8 Well Strips)
CAPZb Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CAPZb Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001193469.1
Nucleotide Accession Num NM_001206540.2
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBRN7
Species Reactivity Human