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96 Wells
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Additional Information

Name CASP5 ELISA Kit (Human) (GWB-KBBCM4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CASP5
Gene id 838
Alias Symbols CASP-5, Caspase-5, ICE(rel)III, ICE(rel)-III, ICEREL-III, ICH3, ICH-3, MGC141966, Protease ICH-3, Protease TY
Swissprot ID P51878
Protein Name Caspase-5
Kit Principle Genway Biotech CASP5 ELISA Kit (Human) (GWB-KBBCM4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CASP5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CASP5 captured in the well.
Kit Component
Anti-CASP5 Microplate96 Wells (12 x 8 Well Strips)
CASP5 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CASP5 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001129581.1
Nucleotide Accession Num NM_001136109.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBCM4
Species Reactivity Human