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Additional Information

Name CASP5 ELISA Kit (Human) (GWB-KBBXJ7)
Description of Target Mediator of programmed cell death (apoptosis).
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.056 ng/mL
Sample Type tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CASP5
Gene id 838
Alias Symbols CASP-5, Caspase-5, ICE(rel)III, ICE(rel)-III, ICEREL-III, ICH3, ICH-3, MGC141966, Protease ICH-3, Protease TY
Swissprot ID P51878
Protein Name caspase-5
Kit Principle Genway Biotech CASP5 ELISA Kit (Human) (GWB-KBBXJ7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CASP5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CASP5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CASP5 captured in the well.
Kit Component
Anti-Sparc Microplate96 Wells (12 x 8 Well Strips)
Sparc Lyophilized Standard2 x 10 ng
100X Biotinylated Sparc Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001129581
Nucleotide Accession Num NM_001136109
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXJ7
Species Reactivity Human