CAV2 ELISA Kit (Human) (GWB-KBBKF9)

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GWB-KBBKF9

96 Wells
$659.00
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Additional Information

Name CAV2 ELISA Kit (Human) (GWB-KBBKF9)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.056 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CAV2
Gene id 858
Alias Symbols CAV, Caveolin-2, MGC12294
Swissprot ID P51636
Protein Name Caveolin-2
Description of Target May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveol
Kit Principle Genway Biotech CAV2 ELISA Kit (Human) (GWB-KBBKF9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CAV2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CAV2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CAV2 captured in the well.
Kit Component
ComponentAmount
Anti-CAV2 Microplate96 Wells (12 x 8 Well Strips)
CAV2 Lyophilized Standard2 x 10 ng
100X Biotinylated CAV2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001193676
Nucleotide Accession Num NM_001206747
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)96-104%83-104%89-103%98-105%
EDTA Plasma (n=5)80-89%93-101%78-93%92-101%
Heparin Plasma (n=5)80-101%95-103%79-101%83-92%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)79-9689
EDTA Plasma (n=5)82-9788
Heparin Plasma (n=5)90-10295
Datasheets/Manuals Printable datasheet for GWB-KBBKF9
Species Reactivity Human