CBLN1 ELISA Kit (Human) (GWB-KBBHS6) (Aviva Cat. No. OKDD00169)

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GWB-KBBHS6

Aviva Cat. No.: OKDD00169

Additional Information

Name CBLN1 ELISA Kit (Human) (GWB-KBBHS6)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type Serum, Plasma, Tissue homogenates,
Kit Detection Absorbance, OD 450 nm
Gene Symbol CBLN1
Gene id 869
Alias Symbols Cerebellin-1, Precerebellin
Swissprot ID P23435
Protein Name cerebellin-1
Description of Target This gene encodes a cerebellum-specific precursor protein, precerebellin, with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives, including the hexadecapeptid
Kit Principle Genway Biotech CBLN1 ELISA Kit (Human) (GWB-KBBHS6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CBLN1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CBLN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CBLN1 captured in the well.
Kit Component
ComponentAmount
Anti-CBLN1 Microplate96 Wells (12 x 8 Well Strips)
CBLN1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CBLN1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004343.1
Nucleotide Accession Num NM_004352.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBHS6
Species Reactivity Human