CBLN1 ELISA Kit (Human) (GWB-KBBLW7) (Aviva Cat. No. OKCD00499)

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Please see item number OKCD00499 to view product details and for ordering information.


GWB-KBBLW7

Aviva Cat. No.: OKCD00499

Additional Information

Name CBLN1 ELISA Kit (Human) (GWB-KBBLW7)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CBLN1
Gene id 869
Alias Symbols Cerebellin-1, Precerebellin
Swissprot ID P23435
Protein Name cerebellin-1
Description of Target Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interactio
Kit Principle Genway Biotech CBLN1 ELISA Kit (Human) (GWB-KBBLW7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CBLN1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CBLN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CBLN1 captured in the well.
Kit Component
ComponentAmount
Anti-Atp1b1 Microplate96 Wells (12 x 8 Well Strips)
Atp1b1 Lyophilized Standard2 x 10 ng
100X Biotinylated Atp1b1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004343
Nucleotide Accession Num NM_004352
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)92-105%87-98%82-94%85-99%
EDTA Plasma (n=5)85-97%93-106%80-96%93-107%
Heparin Plasma (n=5)88-99%83-96%90-103%87-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9689
EDTA Plasma (n=5)85-9993
Heparin Plasma (n=5)91-10396
Datasheets/Manuals Printable datasheet for GWB-KBBLW7
Species Reactivity Human