Ccl11 ELISA Kit (Horse) (GWB-KBBIN1) (Aviva Cat. No. OKCD01129)

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Please see item number OKCD01129 to view product details and for ordering information.


GWB-KBBIN1

Aviva Cat. No.: OKCD01129

Additional Information

Name Ccl11 ELISA Kit (Horse) (GWB-KBBIN1)
Kit Range 62.5 - 4,000 pg/mL
Sensitivity < 22.7 pg/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CCL11
Gene id 100033949
Alias Symbols C-C motif chemokine 11, Eotaxin, SCYA11, Small-inducible cytokine A11
Swissprot ID Q9TTQ4
Protein Name eotaxin
Description of Target In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3 (
Kit Principle Genway Biotech Ccl11 ELISA Kit (Horse) (GWB-KBBIN1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl11 captured in the well.
Kit Component
ComponentAmount
Anti-GSTA1 Microplate96 Wells (12 x 8 Well Strips)
GSTA1 Lyophilized Standard2 x 4 ng
100X Biotinylated GSTA1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_002977
Nucleotide Accession Num NM_002986
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)81-101%90-105%94-105%84-99%
EDTA Plasma (n=5)86-98%83-93%89-103%82-90%
Heparin Plasma (n=5)98-105%81-102%79-96%79-91%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9187
EDTA Plasma (n=5)78-9389
Heparin Plasma (n=5)80-9284
Datasheets/Manuals Printable datasheet for GWB-KBBIN1
Species Reactivity Horse