CCL11 ELISA Kit (Human) (GWB-KBBCF2) (Aviva Cat. No. OKCD00124)

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Please see item number OKCD00124 to view product details and for ordering information.


GWB-KBBCF2

Aviva Cat. No.: OKCD00124

Additional Information

Name CCL11 ELISA Kit (Human) (GWB-KBBCF2)
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 6.4 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CCL11
Gene id 6356
Alias Symbols CCL11, SCYA11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11
Swissprot ID Q6I9T4
Protein Name eotaxin
Description of Target This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies
Kit Principle Genway Biotech CCL11 ELISA Kit (Human) (GWB-KBBCF2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL11 captured in the well.
Kit Component
ComponentAmount
Anti-CCL11 Microplate96 Wells (12 x 8 Well Strips)
CCL11 Lyophilized Standard2 x 1 ng
100X Biotinylated CCL11 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_002977
Nucleotide Accession Num NM_002986
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-88%95-102%97-105%93-101%
EDTA Plasma (n=5)87-98%99-105%93-101%83-104%
Heparin Plasma (n=5)88-96%99-105%87-101%78-95%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-10285
EDTA Plasma (n=5)96-105101
Heparin Plasma (n=5)96-105101
Datasheets/Manuals Printable datasheet for GWB-KBBCF2
Species Reactivity Human