| Name |
CCL11 ELISA Kit (Human) (GWB-KBBCF2) |
| Kit Range |
15.62 - 1,000 pg/mL |
| Sensitivity |
< 6.4 pg/mL |
| Sample Type |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CCL11 |
| Gene id |
6356 |
| Alias Symbols |
CCL11, SCYA11, Eotaxin 1, Eosinophil Chemotactic Protein 1, Chemokine C-C-Motif Ligand 11, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11 |
| Swissprot ID |
Q6I9T4 |
| Protein Name |
eotaxin |
| Description of Target |
This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies |
| Kit Principle |
Genway Biotech CCL11 ELISA Kit (Human) (GWB-KBBCF2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL11 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL11 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL11 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CCL11 Microplate | 96 Wells (12 x 8 Well Strips) | | CCL11 Lyophilized Standard | 2 x 1 ng | | 100X Biotinylated CCL11 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_002977 |
| Nucleotide Accession Num |
NM_002986 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Kit linearity |
| Sample | 1:2 Dilution | 1:4 Dilution | 1:8 Dilution | 1:16 Dilution |
|---|
| Serum (n=5) | 79-88% | 95-102% | 97-105% | 93-101% | |
| EDTA Plasma (n=5) | 87-98% | 99-105% | 93-101% | 83-104% | |
| Heparin Plasma (n=5) | 88-96% | 99-105% | 87-101% | 78-95% | |
|
| Kit Recovery |
| Matrix | Recovery Range (%) | Average (%) |
|---|
| Serum (n=5) | 80-102 | 85 | |
| EDTA Plasma (n=5) | 96-105 | 101 | |
| Heparin Plasma (n=5) | 96-105 | 101 | |
|
| Datasheets/Manuals |
Printable datasheet for GWB-KBBCF2 |
| Species Reactivity |
Human |
