Ccl19 ELISA Kit (Dog) (GWB-KBBKT5) (Aviva Cat. No. OKCD00145)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKCD00145 to view product details and for ordering information.


GWB-KBBKT5

Aviva Cat. No.: OKCD00145

Additional Information

Name Ccl19 ELISA Kit (Dog) (GWB-KBBKT5)
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 5.6 pg/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CCL19
Gene id 448793
Alias Symbols CCL19, CKb11, ELC, MIP3-B, SCYA19, Chemokine C-C-Motif Ligand 19, Beta Chemokine Exodus-3, CK Beta-11, EBI1-Ligand Chemokine, Small Inducible Cytokine Subfamily A19
Swissprot ID Q68AY7
Protein Name C-C motif chemokine 19
Kit Principle Genway Biotech Ccl19 ELISA Kit (Dog) (GWB-KBBKT5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl19 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl19 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl19 captured in the well.
Kit Component
ComponentAmount
Anti-CCL19 Microplate96 Wells (12 x 8 Well Strips)
CCL19 Lyophilized Standard2 x 1 ng
100X Biotinylated CCL19 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001005256
Nucleotide Accession Num NM_001005256
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)94-101%92-99%78-95%89-97%
EDTA Plasma (n=5)94-102%78-91%88-97%82-102%
Heparin Plasma (n=5)97-104%88-96%84-95%86-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)98-105102
EDTA Plasma (n=5)93-10197
Heparin Plasma (n=5)98-105101
Datasheets/Manuals Printable datasheet for GWB-KBBKT5
Species Reactivity Dog