CD200R1 ELISA Kit (Human) (GWB-KBBEQ2)

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96 Wells
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Additional Information

Name CD200R1 ELISA Kit (Human) (GWB-KBBEQ2)
Kit Range 15.6 - 1,000 pg/mL
Sensitivity < 6.6 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CD200R1
Gene id 131450
Alias Symbols CD200 cell surface glycoprotein receptor, CD200R, Cell surface glycoprotein CD200 receptor 1, Cell surface glycoprotein OX2 receptor 1, CRTR2, HCRTR2, MOX2R, OX2R, UNQ2522/PRO6015
Swissprot ID Q8TD46
Protein Name Cell surface glycoprotein CD200 receptor 1
Description of Target Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Al
Kit Principle Genway Biotech CD200R1 ELISA Kit (Human) (GWB-KBBEQ2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD200R1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD200R1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD200R1 captured in the well.
Kit Component
Anti-CD200R1 Microplate96 Wells (12 x 8 Well Strips)
CD200R1 Lyophilized Standard2 x 1 ng
100X Biotinylated CD200R1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_620161
Nucleotide Accession Num NM_138806
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)84-96%80-99%78-97%85-99%
EDTA Plasma (n=5)92-101%97-105%86-97%78-96%
Heparin Plasma (n=5)93-101%93-101%89-101%84-99%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-9087
EDTA Plasma (n=5)79-10190
Heparin Plasma (n=5)87-10496
Datasheets/Manuals Printable datasheet for GWB-KBBEQ2
Species Reactivity Human