Cd300lf ELISA Kit (Mouse) (GWB-KBBVT7)

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96 Wells
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Additional Information

Name Cd300lf ELISA Kit (Mouse) (GWB-KBBVT7)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.62 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CD300LF
Gene id 246746
Alias Symbols CD300 antigen-like family member F, CLIM1, Clm1, CLM1, CLM-1, CMRF35-like molecule 1, Digr2, DIgR2, F730004D16Rik, IgSF13, IREM1, Leukocyte mono-Ig-like receptor 3, Lmir3, LMIR3, MAIR-5, MAIR-V, Myeloid-associated immunoglobulin-like receptor 5, Pigr3
Swissprot ID Q6SJQ7
Protein Name CMRF35-like molecule 1
Description of Target Acts as an inhibitory receptor for myeloid cells and mast cells. Inhibits osteoclast formation. Induces macrophage cell death upon engagement
Kit Principle Genway Biotech Cd300lf ELISA Kit (Mouse) (GWB-KBBVT7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd300lf has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd300lf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd300lf captured in the well.
Kit Component
Anti-MBL Microplate96 Wells (12 x 8 Well Strips)
MBL Lyophilized Standard2 x 100 ng
100X Biotinylated MBL Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001162624
Nucleotide Accession Num NM_001169153
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Kit Recovery
MatrixRecovery Range (%)Average (%)
Datasheets/Manuals Printable datasheet for GWB-KBBVT7
Species Reactivity Mouse