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Cd40 ELISA Kit (Rat) (GWB-KBBYE6)

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Additional Information

Name Cd40 ELISA Kit (Rat) (GWB-KBBYE6)
Description of Target may mediate chronic inflammation including arteriosclerosis; interacts with ligand CD154 and plays a crucial role in humoral and cellular immunity, and in T-cell-mediated inflammatory responses [RGD, Feb 2006]
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 5.2 pg/mL
Sample Type serum, plasma and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CD40
Gene id 171369
Alias Symbols CD40, CDW40, Bp50, P50, B-cell surface antigen CD40, CD40L receptor
Swissprot ID Q4QQW2
Protein Name tumor necrosis factor receptor superfamily member 5
Kit Principle Genway Biotech Cd40 ELISA Kit (Rat) (GWB-KBBYE6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd40 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd40 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd40 captured in the well.
Kit Component
Anti-Cd40 Microplate96 Wells (12 x 8 Well Strips)
Cd40 Lyophilized Standard2 x 1 ng
100X Biotinylated Cd40 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_599187
Nucleotide Accession Num NM_134360
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)80-96%93-105%79-104%96-104%
EDTA Plasma (n=5)82-93%83-92%98-105%99-105%
Heparin Plasma (n=5)94-105%97-105%91-98%78-90%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)91-9996
EDTA Plasma (n=5)93-10198
Heparin Plasma (n=5)79-9086
Datasheets/Manuals Printable datasheet for GWB-KBBYE6
Species Reactivity Rat