CD72 ELISA Kit (Human) (GWB-KBBJB2) (Aviva Cat. No. OKCD00273)

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Please see item number OKCD00273 to view product details and for ordering information.


GWB-KBBJB2

Aviva Cat. No.: OKCD00273

Additional Information

Name CD72 ELISA Kit (Human) (GWB-KBBJB2)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.059 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CD72
Gene id 971
Alias Symbols B-cell differentiation antigen CD72, CD72b, LYB2, Lyb-2
Swissprot ID P21854
Protein Name B-cell differentiation antigen CD72
Description of Target Plays a role in B-cell proliferation and differentiation.
Kit Principle Genway Biotech CD72 ELISA Kit (Human) (GWB-KBBJB2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD72 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD72 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD72 captured in the well.
Kit Component
ComponentAmount
Anti-PPP3CA Microplate96 Wells (12 x 8 Well Strips)
PPP3CA Lyophilized Standard2 x 10 ng
100X Biotinylated PPP3CA Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001773
Nucleotide Accession Num NM_001782
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJB2
Species Reactivity Human