CD99 ELISA Kit (Human) (GWB-KBBKM4)

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96 Wells
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Additional Information

Name CD99 ELISA Kit (Human) (GWB-KBBKM4)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.061 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CD99
Gene id 4267
Alias Symbols 12E7, CD99 antigen, E2 antigen, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, Protein MIC2, T-cell surface glycoprotein E2
Swissprot ID P14209
Protein Name CD99 antigen
Description of Target Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independen
Kit Principle Genway Biotech CD99 ELISA Kit (Human) (GWB-KBBKM4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD99 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD99 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD99 captured in the well.
Kit Component
Anti-CD99 Microplate96 Wells (12 x 8 Well Strips)
CD99 Lyophilized Standard2 x 10 ng
100X Biotinylated CD99 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001116370
Nucleotide Accession Num NM_001122898
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)89-96%82-97%78-94%95-105%
EDTA Plasma (n=5)83-101%89-97%99-105%78-105%
Heparin Plasma (n=5)97-105%85-101%78-98%92-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)82-10586
EDTA Plasma (n=5)80-9187
Heparin Plasma (n=5)80-10391
Datasheets/Manuals Printable datasheet for GWB-KBBKM4
Species Reactivity Human