CDH17 ELISA Kit (Human) (GWB-KBBEX3) (Aviva Cat. No. OKDD00177)

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Please see item number OKDD00177 to view product details and for ordering information.


GWB-KBBEX3

Aviva Cat. No.: OKDD00177

Additional Information

Name CDH17 ELISA Kit (Human) (GWB-KBBEX3)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CDH17
Gene id 1015
Alias Symbols cadherin, Cadherin-17, CDH16, FLJ26931, HPT1, HPT-1, Intestinal peptide-associated transporter HPT-1, LI-cadherin, Liver-intestine cadherin, MGC138218, MGC142024
Swissprot ID Q12864
Protein Name Cadherin-17
Description of Target This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region
Kit Principle Genway Biotech CDH17 ELISA Kit (Human) (GWB-KBBEX3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDH17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDH17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDH17 captured in the well.
Kit Component
ComponentAmount
Anti-CDH17 Microplate96 Wells (12 x 8 Well Strips)
CDH17 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CDH17 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001138135.1
Nucleotide Accession Num NM_001144663.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBEX3
Species Reactivity Human