| Name |
CDH17 ELISA Kit (Human) (GWB-KBBEX3) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
< 0.054 ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates, cell lysates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
CDH17 |
| Gene id |
1015 |
| Alias Symbols |
cadherin, Cadherin-17, CDH16, FLJ26931, HPT1, HPT-1, Intestinal peptide-associated transporter HPT-1, LI-cadherin, Liver-intestine cadherin, MGC138218, MGC142024 |
| Swissprot ID |
Q12864 |
| Protein Name |
Cadherin-17 |
| Description of Target |
This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region |
| Kit Principle |
Genway Biotech CDH17 ELISA Kit (Human) (GWB-KBBEX3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDH17 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDH17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDH17 captured in the well. |
| Kit Component |
| Component | Amount |
| Anti-CDH17 Microplate | 96 Wells (12 x 8 Well Strips) |
| CDH17 Lyophlized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X CDH17 Biotinylated Detector Antibody | 1 x 120 µL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 µL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 10X Sample Diluent | 1 x 30 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL | |
| Protein Accession Num |
NP_001138135.1 |
| Nucleotide Accession Num |
NM_001144663.1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBEX3 |
| Species Reactivity |
Human |
