CDH17 ELISA Kit (Human) (GWB-KBBKG5)

GWB-KBBKG5

96 Wells
$580.00
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Additional Information

Name CDH17 ELISA Kit (Human) (GWB-KBBKG5)
Kit Range 31.25 - 2,000 pg/mL
Sensitivity < 11.6 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CDH17
Gene id 1015
Alias Symbols cadherin, Cadherin-17, CDH16, FLJ26931, HPT1, HPT-1, Intestinal peptide-associated transporter HPT-1, LI-cadherin, Liver-intestine cadherin, MGC138218, MGC142024
Swissprot ID Q12864
Protein Name Cadherin-17
Description of Target Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the m
Kit Principle Genway Biotech CDH17 ELISA Kit (Human) (GWB-KBBKG5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDH17 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDH17 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDH17 captured in the well.
Kit Component
ComponentAmount
Anti-BAI1 Microplate96 Wells (12 x 8 Well Strips)
BAI1 Lyophilized Standard2 x 2 ng
100X Biotinylated BAI1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001138135
Nucleotide Accession Num NM_001144663
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-97%82-104%79-95%94-102%
EDTA Plasma (n=5)83-91%96-104%78-99%85-92%
Heparin Plasma (n=5)82-93%81-98%88-96%78-88%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)90-9794
EDTA Plasma (n=5)80-10288
Heparin Plasma (n=5)96-10399
Datasheets/Manuals Printable datasheet for GWB-KBBKG5