CDK6 ELISA Kit (Human) (GWB-KBBWM5) (Aviva Cat. No. OKDD00179)

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GWB-KBBWM5

Aviva Cat. No.: OKDD00179

Additional Information

Name CDK6 ELISA Kit (Human) (GWB-KBBWM5)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.053 ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CDK6
Gene id 1021
Alias Symbols Cell division protein kinase 6, Cyclin-dependent kinase 6, MGC59692, PLSTIRE, Serine/threonine-protein kinase PLSTIRE
Swissprot ID Q00534
Protein Name Cyclin-dependent kinase 6
Kit Principle Genway Biotech CDK6 ELISA Kit (Human) (GWB-KBBWM5) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDK6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDK6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDK6 captured in the well.
Kit Component
ComponentAmount
Anti-CDK6 Microplate96 Wells (12 x 8 Well Strips)
CDK6 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CDK6 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001138778.1
Nucleotide Accession Num NM_001145306.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBWM5
Species Reactivity Human