CDK8 ELISA Kit (Human) (GWB-KBBNS4) (Aviva Cat. No. OKCD01749)

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GWB-KBBNS4

Aviva Cat. No.: OKCD01749

Additional Information

Name CDK8 ELISA Kit (Human) (GWB-KBBNS4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.127 ng/mL
Sample Type tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CDK8
Gene id 1024
Alias Symbols Cell division protein kinase 8, Cyclin-dependent kinase 8, K35, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8, MGC126074, MGC126075, Protein kinase K35
Swissprot ID P49336
Protein Name Cyclin-dependent kinase 8
Description of Target Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymer
Kit Principle Genway Biotech CDK8 ELISA Kit (Human) (GWB-KBBNS4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDK8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDK8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDK8 captured in the well.
Kit Component
ComponentAmount
Anti-CDK8 Microplate96 Wells (12 x 8 Well Strips)
CDK8 Lyophilized Standard2 x 20 ng
100X Biotinylated CDK8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001251
Nucleotide Accession Num NM_001260
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBNS4
Species Reactivity Human