| Name |
CDK8 ELISA Kit (Mouse) (GWB-KBBJE2) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
<0.053ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
CDK8 |
| Gene id |
264064 |
| Alias Symbols |
Cell division protein kinase 8, Cyclin-dependent kinase 8, Mediator complex subunit CDK8, Mediator of RNA polymerase II transcription subunit CDK8 |
| Swissprot ID |
Q8R3L8 |
| Protein Name |
cyclin-dependent kinase 8 |
| Description of Target |
Component of the mediator complex, a coactivator involved in regulated gene transcription of nearly all rna polymerase ii-dependent genes. mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal rna polymer |
| Kit Principle |
Genway Biotech CDK8 ELISA Kit (Mouse) (GWB-KBBJE2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDK8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDK8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDK8 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CDK8 Microplate | 96 Wells (12 x 8 Well Strips) |
| CDK8 Lyophilized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X CDK8 Biotinylated Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_705827.2 |
| Nucleotide Accession Num |
NM_153599.3 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBJE2 |
| Species Reactivity |
Mouse |
