CDNF ELISA Kit (Human) (GWB-KBBCV7) (Aviva Cat. No. OKCD01915)

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Please see item number OKCD01915 to view product details and for ordering information.


GWB-KBBCV7

Aviva Cat. No.: OKCD01915

Additional Information

Name CDNF ELISA Kit (Human) (GWB-KBBCV7)
Kit Range 15.6 - 1,000 pg/mL
Sensitivity < 6.4 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CDNF
Gene id 441549
Alias Symbols ARMETL1, ARMET-like protein 1, Cerebral dopamine neurotrophic factor, Conserved dopamine neurotrophic factor
Swissprot ID Q49AH0
Protein Name Cerebral dopamine neurotrophic factor
Description of Target Trophic factor for dopamine neurons. Prevents the 6-hydroxydopamine (6-OHDA)-induced degeneration of dopaminergic neurons. When administered after 6-OHDA-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons i
Kit Principle Genway Biotech CDNF ELISA Kit (Human) (GWB-KBBCV7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDNF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDNF captured in the well.
Kit Component
ComponentAmount
Anti-CDNF Microplate96 Wells (12 x 8 Well Strips)
CDNF Lyophilized Standard2 x 1 ng
100X Biotinylated CDNF Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001025125
Nucleotide Accession Num NM_001029954
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-95%84-99%78-101%87-102%
EDTA Plasma (n=5)78-101%83-90%78-101%80-104%
Heparin Plasma (n=5)92-101%87-95%90-98%82-90%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)89-10196
EDTA Plasma (n=5)84-10187
Heparin Plasma (n=5)79-8885
Datasheets/Manuals Printable datasheet for GWB-KBBCV7
Species Reactivity Human