| Name |
CDNF ELISA Kit (Rat) (GWB-KBBQI6) |
| Kit Range |
0.156-10 ng/mL |
| Sensitivity |
<0.067ng/mL |
| Sample Type |
Serum, Plasma, Tissue homogenates |
| Kit Detection |
Absorbance, OD 450 nm |
| Gene Symbol |
CDNF |
| Gene id |
361276 |
| Alias Symbols |
Arginine-rich protein mutated in early stage tumors-like 1, Armetl1, ARMET-like protein 1, Cerebral dopamine neurotrophic factor, Conserved dopamine neurotrophic factor |
| Swissprot ID |
P0C5I0 |
| Protein Name |
cerebral dopamine neurotrophic factor |
| Description of Target |
Trophic factor for dopamine neurons. prevents the 6-hydroxydopamine (6-ohda)-induced degeneration of dopaminergic neurons. when administered after 6-ohda-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons i |
| Kit Principle |
Genway Biotech CDNF ELISA Kit (Rat) (GWB-KBBQI6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDNF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDNF captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CDNF Microplate | 96 Wells (12 x 8 Well Strips) |
| CDNF Lyophilized Standard | 2 x 10 ng |
| Standard Diluent | 1 x 20 mL |
| 100X CDNF Biotinylated Detector Antibody | 1 x 120 uL |
| 100X Avidin-HRP Detection Conjugate | 1 x 120 uL |
| Detector Antibody Diluent | 1 x 12 mL |
| Detection Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001032632.1 |
| Nucleotide Accession Num |
NM_001037543.1 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3.5 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBQI6 |
| Species Reactivity |
Rat |
