CDNF ELISA Kit (Rat) (GWB-KBBQI6) (Aviva Cat. No. OKDD00756)

This exact product is available to purchase through Aviva Systems Biology.
Please see item number OKDD00756 to view product details and for ordering information.


GWB-KBBQI6

Aviva Cat. No.: OKDD00756

Additional Information

Name CDNF ELISA Kit (Rat) (GWB-KBBQI6)
Kit Range 0.156-10 ng/mL
Sensitivity <0.067ng/mL
Sample Type Serum, Plasma, Tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CDNF
Gene id 361276
Alias Symbols Arginine-rich protein mutated in early stage tumors-like 1, Armetl1, ARMET-like protein 1, Cerebral dopamine neurotrophic factor, Conserved dopamine neurotrophic factor
Swissprot ID P0C5I0
Protein Name cerebral dopamine neurotrophic factor
Description of Target Trophic factor for dopamine neurons. prevents the 6-hydroxydopamine (6-ohda)-induced degeneration of dopaminergic neurons. when administered after 6-ohda-lesioning, restores the dopaminergic function and prevents the degeneration of dopaminergic neurons i
Kit Principle Genway Biotech CDNF ELISA Kit (Rat) (GWB-KBBQI6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDNF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDNF captured in the well.
Kit Component
ComponentAmount
Anti-CDNF Microplate96 Wells (12 x 8 Well Strips)
CDNF Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CDNF Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001032632.1
Nucleotide Accession Num NM_001037543.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBQI6
Species Reactivity Rat