CDSN ELISA Kit (Human) (GWB-KBBGS4) (Aviva Cat. No. OKDD00180)

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Please see item number OKDD00180 to view product details and for ordering information.


GWB-KBBGS4

Aviva Cat. No.: OKDD00180

Additional Information

Name CDSN ELISA Kit (Human) (GWB-KBBGS4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.038 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, cell lysates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CDSN
Gene id 1041
Alias Symbols Corneodesmosin, D6S586E, HTSS, S protein
Swissprot ID Q15517
Protein Name Corneodesmosin
Description of Target This gene encodes a protein found in corneodesmosomes, which localize to human epidermis and other cornified squamous epithelia. The encoded protein undergoes a series of cleavages during corneocyte maturation. This gene is highly polymorphic in human pop
Kit Principle Genway Biotech CDSN ELISA Kit (Human) (GWB-KBBGS4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDSN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDSN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDSN captured in the well.
Kit Component
ComponentAmount
Anti-CDSN Microplate96 Wells (12 x 8 Well Strips)
CDSN Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CDSN Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001255.3
Nucleotide Accession Num NM_001264.4
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBGS4
Species Reactivity Human