CDX2 ELISA Kit (Human) (GWB-KBBNH3) (Aviva Cat. No. OKCD01666)

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Please see item number OKCD01666 to view product details and for ordering information.


GWB-KBBNH3

Aviva Cat. No.: OKCD01666

Additional Information

Name CDX2 ELISA Kit (Human) (GWB-KBBNH3)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CDX2
Gene id 1045
Alias Symbols Caudal-type homeobox protein 2, CDX3, CDX-3, Homeobox protein CDX-2
Swissprot ID Q99626
Protein Name Homeobox protein CDX-2
Description of Target Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.
Kit Principle Genway Biotech CDX2 ELISA Kit (Human) (GWB-KBBNH3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CDX2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CDX2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CDX2 captured in the well.
Kit Component
ComponentAmount
Anti-CDX2 Microplate96 Wells (12 x 8 Well Strips)
CDX2 Lyophilized Standard2 x 10 ng
100X Biotinylated CDX2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001256
Nucleotide Accession Num NM_001265
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBNH3
Species Reactivity Human