CEACAM8 ELISA Kit (Human) (GWB-KBBUS9) (Aviva Cat. No. OKCD01729)

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Please see item number OKCD01729 to view product details and for ordering information.


Aviva Cat. No.: OKCD01729

Additional Information

Kit Range 15.6 - 1,000 pg/mL
Sensitivity < 5.8 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CEACAM8
Gene id 1088
Alias Symbols Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CD66b, CD67, CD67 antigen, CGM6, NCA-95, Non-specific cross-reacting antigen NCA-95
Swissprot ID P31997
Protein Name Carcinoembryonic antigen-related cell adhesion molecule 8
Kit Principle Genway Biotech CEACAM8 ELISA Kit (Human) (GWB-KBBUS9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CEACAM8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CEACAM8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CEACAM8 captured in the well.
Kit Component
Anti-CEACAM8 Microplate96 Wells (12 x 8 Well Strips)
CEACAM8 Lyophilized Standard2 x 1 ng
100X Biotinylated CEACAM8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001807
Nucleotide Accession Num NM_001816
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)94-105%91-101%78-88%91-98%
EDTA Plasma (n=5)78-92%97-105%92-101%82-89%
Heparin Plasma (n=5)99-105%83-97%98-105%98-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)86-9995
EDTA Plasma (n=5)85-10596
Heparin Plasma (n=5)97-104101
Datasheets/Manuals Printable datasheet for GWB-KBBUS9
Species Reactivity Human