CENPE ELISA Kit (Human) (GWB-KBBBD2) (Aviva Cat. No. OKCD00791)

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GWB-KBBBD2

Aviva Cat. No.: OKCD00791

Additional Information

Name CENPE ELISA Kit (Human) (GWB-KBBBD2)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.124 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CENPE
Gene id 1062
Alias Symbols CENP-E, Centromere-associated protein E, Centromere protein E, KIF10, Kinesin-related protein CENPE
Swissprot ID Q02224
Protein Name Centromere-associated protein E
Description of Target Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor who
Kit Principle Genway Biotech CENPE ELISA Kit (Human) (GWB-KBBBD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CENPE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CENPE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CENPE captured in the well.
Kit Component
ComponentAmount
Anti-XRCC6 Microplate96 Wells (12 x 8 Well Strips)
XRCC6 Lyophilized Standard2 x 20 ng
100X Biotinylated XRCC6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001273663
Nucleotide Accession Num NM_001286734
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBBD2
Species Reactivity Human