Name |
CENPE ELISA Kit (Human) (GWB-KBBBD2) |
Kit Range |
0.312 - 20 ng/mL |
Sensitivity |
< 0.124 ng/mL |
Sample Type |
tissue homogenates, cell lysates and other biological fluids. |
Kit Detection |
Colorimetric, OD 450 nm |
Gene Symbol |
CENPE |
Gene id |
1062 |
Alias Symbols |
CENP-E, Centromere-associated protein E, Centromere protein E, KIF10, Kinesin-related protein CENPE |
Swissprot ID |
Q02224 |
Protein Name |
Centromere-associated protein E |
Description of Target |
Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end-directed motor who |
Kit Principle |
Genway Biotech CENPE ELISA Kit (Human) (GWB-KBBBD2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CENPE has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CENPE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CENPE captured in the well. |
Kit Component |
Component | Amount |
---|
Anti-XRCC6 Microplate | 96 Wells (12 x 8 Well Strips) | XRCC6 Lyophilized Standard | 2 x 20 ng | 100X Biotinylated XRCC6 Detector Antibody | 120 uL | Avidin/HRP Conjugate | 120 uL | Standard Diluent | 1 x 20 mL | Detector Antibody Diluent | 1 x 12 mL | Conjugate Diluent | 1 x 12 mL | 30X Wash Buffer | 1 x 20 mL | TMB Substrate | 1 x 9 mL | Stop Solution | 1 x 6 mL |
|
Protein Accession Num |
NP_001273663 |
Nucleotide Accession Num |
NM_001286734 |
Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
Kit Duration |
~ 3 Hours |
Datasheets/Manuals |
Printable datasheet for GWB-KBBBD2 |
Species Reactivity |
Human |