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96 Wells
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Additional Information

Name CFHR3 ELISA Kit (Human) (GWB-KBBLZ4)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.057 ng/mL
Sample Type Serum, Plasma
Kit Detection Absorbance, OD 450 nm
Gene Symbol CFHR3
Gene id 10878
Alias Symbols CFHL3, Complement factor H-related protein 3, DOWN16, FHR3, FHR-3, H factor-like protein 3, HLF4
Swissprot ID Q02985
Protein Name Complement factor H-related protein 3
Description of Target The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-re
Kit Principle Genway Biotech CFHR3 ELISA Kit (Human) (GWB-KBBLZ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFHR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFHR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFHR3 captured in the well.
Kit Component
Anti-CFHR3 Microplate96 Wells (12 x 8 Well Strips)
CFHR3 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CFHR3 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001160096.1
Nucleotide Accession Num NM_001166624.1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBLZ4
Species Reactivity Human