CFHR5 ELISA Kit (Human) (GWB-KBBIK2)

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GWB-KBBIK2

96 Wells
$729.00
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Additional Information

Name CFHR5 ELISA Kit (Human) (GWB-KBBIK2)
Kit Range 31.2 - 2,000 pg/mL
Sensitivity < 14.8 pg/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CFHR5
Gene id 81494
Alias Symbols CFHL5, Complement factor H-related protein 5, FHR5, FHR-5, FLJ10549, MGC133240
Swissprot ID Q9BXR6
Protein Name Complement factor H-related protein 5
Description of Target Involved in complement regulation. The dimerized forms have avidity for tissue-bound complement fragments and efficiently compete with the physiological complement inhibitor CFH.
Kit Principle Genway Biotech CFHR5 ELISA Kit (Human) (GWB-KBBIK2) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CFHR5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CFHR5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CFHR5 captured in the well.
Kit Component
ComponentAmount
Anti-CFHR5 Microplate96 Wells (12 x 8 Well Strips)
CFHR5 Lyophilized Standard2 x 2 ng
100X Biotinylated CFHR5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_110414
Nucleotide Accession Num NM_030787.
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-104%79-97%78-93%78-103%
EDTA Plasma (n=5)83-101%81-102%98-105%89-96%
Heparin Plasma (n=5)97-104%92-101%79-94%89-97%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)88-103101
EDTA Plasma (n=5)88-9995
Heparin Plasma (n=5)80-9588
Datasheets/Manuals Printable datasheet for GWB-KBBIK2
Species Reactivity Human