Chgb ELISA Kit (Mouse) (GWB-KBBQS4) (Aviva Cat. No. OKCD00887)

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Please see item number OKCD00887 to view product details and for ordering information.


GWB-KBBQS4

Aviva Cat. No.: OKCD00887

Additional Information

Name Chgb ELISA Kit (Mouse) (GWB-KBBQS4)
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.118 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CHGB
Gene id 12653
Alias Symbols CgB, Chromogranin-B, Scg1, Scg-1, Secretogranin-1, Secretogranin I, secretogranin I, SgI
Swissprot ID P16014
Protein Name secretogranin-1
Description of Target Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
Kit Principle Genway Biotech Chgb ELISA Kit (Mouse) (GWB-KBBQS4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Chgb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Chgb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Chgb captured in the well.
Kit Component
ComponentAmount
Anti-IARS Microplate96 Wells (12 x 8 Well Strips)
IARS Lyophilized Standard2 x 20 ng
100X Biotinylated IARS Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_031720
Nucleotide Accession Num NM_007694
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)78-97%89-103%88-103%86-101%
EDTA Plasma (n=5)80-101%90-98%81-96%99-105%
Heparin Plasma (n=5)94-105%79-89%80-89%93-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)83-10187
EDTA Plasma (n=5)82-9286
Heparin Plasma (n=5)89-9793
Datasheets/Manuals Printable datasheet for GWB-KBBQS4
Species Reactivity Mouse