Description
Short Description: AGP is a 52 kDA serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, AGP is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of AGP include AGP deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases. Genetic variants of AGP do occur.
Additional Information
| Name | Chicken Alpha 1-Acid Glycoprotein (AGP) ELISA (GWB-374Z11) | ||||||||||||||||
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| Related Product Names | Orosomucoid, Orosomucoid-1, OMD-1, AGP-1, AGP, Alpha-1-acid glycoprotein, Chicken Orosomucoid ELISA Kit | ||||||||||||||||
| Kit Range | 6.25 ng/ml - 200 ng/ml | ||||||||||||||||
| Sensitivity | 1.975 ng/ml | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Gene Symbol | ORM1 | ||||||||||||||||
| Gene Fullname | orosomucoid 1 (ovoglycoprotein) | ||||||||||||||||
| Gene id | 395220 | ||||||||||||||||
| Alias Symbols | OGCHI | ||||||||||||||||
| Swissprot ID | Q8JIG5 | ||||||||||||||||
| Protein Name | Alpha-1-acid glycoprotein | ||||||||||||||||
| Description of Target | Functions as transport protein in the blood stream. | ||||||||||||||||
| Kit Principle | Genway Biotech Alpha 1-Acid Glycoprotein ELISA Kit (Chicken) (GWB-374Z11) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-acid glycoprotein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-acid glycoprotein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-acid glycoprotein captured in the well. | ||||||||||||||||
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| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Homology | Chicken | ||||||||||||||||
| Standard Range | 200 ng/ml-3.125 ng/ml | ||||||||||||||||
| Product Format | In Solution | ||||||||||||||||
| Specimen/Volumes | Refer To Manual | ||||||||||||||||
| Concentration | Lot Specific | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-AGP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-CHICKEN AGPELISA MICRO PLATE Anti-Chicken AGP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. CHICKEN AGP CALIBRATOR Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
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| Reactivity | Chicken | ||||||||||||||||
| Kit Size | 96 well | ||||||||||||||||
| Kit Duration | 70 Minutes | ||||||||||||||||
| Datasheets/Manuals | Printable datasheet for GWB-374Z11 | ||||||||||||||||
| Storage | Refer to kit instructions for component storage | ||||||||||||||||
| Species Reactivity | Chicken | ||||||||||||||||
| Lead Time | Domestic: within 1 week delivery | International: 1 week | ||||||||||||||||
| Intended Use | Research Use Only | ||||||||||||||||
| Additional Information | Intended Use: The AGP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP in biological fluid of chickens. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Chicken AGP antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-CHICKEN AGP ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Chicken AGP. 7. CHICKEN AGP CALIBRATOR One vial containing a Chicken AGP calibrator. |