Chicken Alpha 1-Acid Glycoprotein (AGP) ELISA (GWB-374Z11)

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GWB-374Z11

96 Wells
$469.00
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Description

Short Description: AGP is a 52 kDA serine protease inhibitor (serpin) in blood, which protects tissue from enzymes from inflammatory cells, especially elastase. In certain acute phase inflammatory reactions, AGP is elevated in order to limit the damage caused by activated neutrophil granulocytes and their enzyme elastase. Disorders of AGP include AGP deficiency, a hereditary disorder that can lead to severe tissue breakdown during inflammation. This may result in pulmonary emphysema and liver cirrhosis, in severe cases. Genetic variants of AGP do occur.

Additional Information

Name Chicken Alpha 1-Acid Glycoprotein (AGP) ELISA (GWB-374Z11)
Related Product Names Orosomucoid, Orosomucoid-1, OMD-1, AGP-1, AGP, Alpha-1-acid glycoprotein, Chicken Orosomucoid ELISA Kit
Kit Range 6.25 ng/ml - 200 ng/ml
Sensitivity 1.975 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol ORM1
Gene Fullname orosomucoid 1 (ovoglycoprotein)
Gene id 395220
Alias Symbols OGCHI
Swissprot ID Q8JIG5
Protein Name Alpha-1-acid glycoprotein
Description of Target Functions as transport protein in the blood stream.
Kit Principle Genway Biotech Alpha 1-Acid Glycoprotein ELISA Kit (Chicken) (GWB-374Z11) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Alpha-1-acid glycoprotein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Alpha-1-acid glycoprotein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Alpha-1-acid glycoprotein captured in the well.
Kit Component
ComponentAmount
Anti-Chicken Alpha 1-Acid Glycoprotein Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Chicken Alpha 1-Acid Glycoprotein Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Chicken
Standard Range 200 ng/ml-3.125 ng/ml
Product Format In Solution
Specimen/Volumes Refer To Manual
Concentration Lot Specific
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-AGP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-CHICKEN AGPELISA MICRO PLATE
Anti-Chicken AGP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. CHICKEN AGP CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Chicken
Kit Size 96 well
Kit Duration 70 Minutes
Datasheets/Manuals Printable datasheet for GWB-374Z11
Storage Refer to kit instructions for component storage
Species Reactivity Chicken
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information Intended Use: The AGP test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring AGP in biological fluid of chickens.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Chicken AGP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-CHICKEN AGP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Chicken AGP.
7. CHICKEN AGP CALIBRATOR
One vial containing a Chicken AGP calibrator.