Chikungunya Virus IgM μ-capture ELISA (GWB-7CF864)



96 Wells
Add to Cart


Product Name Chikungunya Virus IgM μ-capture ELISA
# of Samples 1 x 96 Assays
Intended Use The Chikungunya IgM μ-capture ELISA is intended for the qualitative determination of IgM class antibodies to Chikungunya virus in human serum and plasma (citrate).
Introduction Chikungunya virus is an arthropod borne virus of the genus Alphavirus (family Togaviridae). The Alphavirus genus contains at least 24 distinct species. These are lipid-enveloped virions with a diameter of 50 to 60 nm.
Alphavirus infections are initiated by the bite of an infected mosquito, which results in the deposition of virus in subcutaneous and possibly cutaneous tissues. After an incubation period of 1 to 12 days the Chikungunya fever develops.
Chikungunya fever (Chikungunya means that which bends up, in reference to the crippling manifestations of the disease) is an acute viral infection characterized by a rapid transition from a state of good health to illness that includes severe arthralgia and fever.
Temperature rises abruptly to as high as 40C and is often accompanied by shaking chills. After a few days, fever may abate and recrudesce, giving rise to a sddleback fever curve. Arthralgia is polyarticular, favoring the small joints and sites of previous injuries, and is most intense on arising. Patients typically avoid movement as much as possible. Joints may swell without significant fluid accumulations. These symptoms may last from 1 week to several months and are accompanied by myalgia. The rash characteristically appears on the first day of illness, but onset may be delayed. It usually arises as a flush over the face and neck, which evolves to a maculopapular or macular form that may be pruritic. The latter lesions appear on the trunk, limbs, face, plams and soles, in that order of frequency. Petechial skin lesions have also been noted. Headache, photophobia, retro-orbitral pain, sore throat with objective signs of pharyngitis, nausea and vomiting also occur in this setting. Occasionally, however persistent arthralgia and polyarthritis (lasting months or even years) do occur, sometimes involving joint destruction. Even rarer, sequelae include encephalitis and meningoencephalitis with high lethality rates.
The virus has major importance in Africa and Asia. From 20% to more than 90% of the population of tropical and subtropical show serologic evidence of infection. Because Aedes mosquitoes are increasingly prevalent in North Africa and South America, where the population would be uniformly susceptible to infection, the possibility for epidemics is evident. Chikungunya virus infections are imported to central Europe mainly by travellers to tropical and subtropical countries.
The presence of virus resp. infection may be identified by
Serology: Detection of antibodies by IF, ELISA.
Principles of the assay The qualitative immunoenzymatic determination of IgM-class antibodies to Chikungunya is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.
Microtiter strip wells are precoated with anti human IgM to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample and control material Chikungunya antigen solution is added. After a further washing step a mixture of biotinylated Chikungunya antibody and Streptavidin conjugate is added. After washing the captured Chikungunya-specific immuncomplex is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Chikungunya-specific IgM antibodies in the patient specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability The reagents are stable up to the expiry date stated on the label when stored at 2-8C.
References Gerald L. Mandell, John E. Bennett, Raphael Dolin, Principles and practice of Infectious diseases, 2005, Chapter 147, 1913-1919 Patrick Hochedez, Staphane Jaureguiberry, Monique Debruyne, Philippe Bossi, Pierre Hausfater, Gilles Brucker, Francois Bricaire, Eric Caumes, Chikungunya infection in travellers, Emerging Infectios Diseases Vol. 12, No. 10, October 2006, 1565-1566F.H. Kayser, K.A. Bienz, J. Eckert, R.M. Zinkernagel, Medical Microbiology, Stuttgart 2005, 440-441.

Additional Information

Name Chikungunya Virus IgM μ-capture ELISA (GWB-7CF864)
Related Product Names Chikungunya Virus IgM μ-capture ELISA
Datasheets/Manuals Printable datasheet for GWB-7CF864
Storage The reagents are stable up to the expiry date stated on the label when stored at 2...8 C.
Intended Use Research Use Only