CHL1 ELISA Kit (Human) (GWB-KBBKV1)

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GWB-KBBKV1

96 Wells
$729.00
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Additional Information

Name CHL1 ELISA Kit (Human) (GWB-KBBKV1)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CHL1
Gene id 10752
Alias Symbols CALL, Close homolog of L1, FLJ44930, L1CAM2, MGC132578, Neural cell adhesion molecule L1-like protein
Swissprot ID O00533
Protein Name neural cell adhesion molecule L1-like protein
Description of Target Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death.
Kit Principle Genway Biotech CHL1 ELISA Kit (Human) (GWB-KBBKV1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHL1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHL1 captured in the well.
Kit Component
ComponentAmount
Anti-ABCC10 Microplate96 Wells (12 x 8 Well Strips)
ABCC10 Lyophilized Standard2 x 10 ng
100X Biotinylated ABCC10 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001240316
Nucleotide Accession Num NM_001253387
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
80-101%89-96%78-102%80-94%
Kit Recovery
MatrixRecovery Range (%)Average (%)
92-10397
Datasheets/Manuals Printable datasheet for GWB-KBBKV1
Species Reactivity Human