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Additional Information

Name CHRNA3 ELISA Kit (Human) (GWB-KBBGG8)
Description of Target After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.107 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CHRNA3
Gene id 1136
Alias Symbols LNCR2, MGC104879, NACHRA3, Neuronal acetylcholine receptor subunit alpha-3, PAOD2
Swissprot ID P32297
Protein Name neuronal acetylcholine receptor subunit alpha-3
Kit Principle Genway Biotech CHRNA3 ELISA Kit (Human) (GWB-KBBGG8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRNA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRNA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRNA3 captured in the well.
Kit Component
Anti-SI Microplate96 Wells (12 x 8 Well Strips)
SI Lyophilized Standard2 x 20 ng
100X Biotinylated SI Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000734
Nucleotide Accession Num NM_000743
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBGG8
Species Reactivity Human