| Name |
CHRNA7 ELISA Kit (Human) (GWB-KBBPU8) |
| Description of Target |
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin. |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.125 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CHRNA7 |
| Gene id |
1139 |
| Alias Symbols |
CHRNA7-2, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7 |
| Swissprot ID |
P36544 |
| Protein Name |
Neuronal acetylcholine receptor subunit alpha-7 |
| Kit Principle |
Genway Biotech CHRNA7 ELISA Kit (Human) (GWB-KBBPU8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRNA7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRNA7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRNA7 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CYLD Microplate | 96 Wells (12 x 8 Well Strips) | | CYLD Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated CYLD Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_000737 |
| Nucleotide Accession Num |
NM_000746 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBPU8 |
| Species Reactivity |
Human |
