CHRNA7 ELISA Kit (Human) (GWB-KBBPU8)

GWB-KBBPU8

96 Wells
$580.00
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Additional Information

Name CHRNA7 ELISA Kit (Human) (GWB-KBBPU8)
Availability In Stock
Kit Range 0.312 - 20 ng/mL
Sensitivity < 0.125 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CHRNA7
Gene id 1139
Alias Symbols CHRNA7-2, NACHRA7, Neuronal acetylcholine receptor subunit alpha-7
Swissprot ID P36544
Protein Name Neuronal acetylcholine receptor subunit alpha-7
Description of Target After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
Kit Principle Genway Biotech CHRNA7 ELISA Kit (Human) (GWB-KBBPU8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CHRNA7 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CHRNA7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CHRNA7 captured in the well.
Kit Component
ComponentAmount
Anti-CYLD Microplate96 Wells (12 x 8 Well Strips)
CYLD Lyophilized Standard2 x 20 ng
100X Biotinylated CYLD Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_000737
Nucleotide Accession Num NM_000746
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBPU8
Species Reactivity Human