CLDN5 ELISA Kit (Human) (GWB-KBBDM6) (Aviva Cat. No. OKCD01861)

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Please see item number OKCD01861 to view product details and for ordering information.


GWB-KBBDM6

Aviva Cat. No.: OKCD01861

Additional Information

Name CLDN5 ELISA Kit (Human) (GWB-KBBDM6)
Kit Range 31.2 - 2,000 pg/mL
Sensitivity < 13.5 pg/mL
Sample Type serum, plasma, tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CLDN5
Gene id 7122
Alias Symbols AWAL, BEC1, Claudin-5, CPETRL1, TMDVCF, TMVCF, Transmembrane protein deleted in VCFS
Swissprot ID O00501
Protein Name Claudin-5
Description of Target Plays a major role in tight junction-specific obliteration of the intercellular space.
Kit Principle Genway Biotech CLDN5 ELISA Kit (Human) (GWB-KBBDM6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN5 captured in the well.
Kit Component
ComponentAmount
Anti-CLDN5 Microplate96 Wells (12 x 8 Well Strips)
CLDN5 Lyophilized Standard2 x 2 ng
100X Biotinylated CLDN5 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001124333
Nucleotide Accession Num NM_001130861
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)99-105%86-104%93-101%81-89%
EDTA Plasma (n=5)87-101%94-101%90-99%78-89%
Heparin Plasma (n=5)78-104%81-98%78-96%87-103%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)97-104101
EDTA Plasma (n=5)81-9188
Heparin Plasma (n=5)96-103101
Datasheets/Manuals Printable datasheet for GWB-KBBDM6
Species Reactivity Human