CLDN8 ELISA Kit (Human) (GWB-KBBSW8) (Aviva Cat. No. OKCD01865)

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Please see item number OKCD01865 to view product details and for ordering information.


GWB-KBBSW8

Aviva Cat. No.: OKCD01865

Additional Information

Name CLDN8 ELISA Kit (Human) (GWB-KBBSW8)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.055 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CLDN8
Gene id 9073
Alias Symbols Claudin-8, UNQ779/PRO1573
Swissprot ID P56748
Protein Name Claudin-8
Description of Target Plays a major role in tight junction-specific obliteration of the intercellular space.
Kit Principle Genway Biotech CLDN8 ELISA Kit (Human) (GWB-KBBSW8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN8 captured in the well.
Kit Component
ComponentAmount
Anti-CLDN8 Microplate96 Wells (12 x 8 Well Strips)
CLDN8 Lyophilized Standard2 x 10 ng
100X Biotinylated CLDN8 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_955360
Nucleotide Accession Num NM_199328
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBSW8
Species Reactivity Human