| Name |
CLDN8 ELISA Kit (Human) (GWB-KBBSW8) |
| Kit Range |
0.156 - 10 ng/mL |
| Sensitivity |
< 0.055 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CLDN8 |
| Gene id |
9073 |
| Alias Symbols |
Claudin-8, UNQ779/PRO1573 |
| Swissprot ID |
P56748 |
| Protein Name |
Claudin-8 |
| Description of Target |
Plays a major role in tight junction-specific obliteration of the intercellular space. |
| Kit Principle |
Genway Biotech CLDN8 ELISA Kit (Human) (GWB-KBBSW8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLDN8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLDN8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLDN8 captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-CLDN8 Microplate | 96 Wells (12 x 8 Well Strips) |
| CLDN8 Lyophilized Standard | 2 x 10 ng |
| 100X Biotinylated CLDN8 Detector Antibody | 120 uL |
| Avidin/HRP Conjugate | 120 uL |
| Standard Diluent | 1 x 20 mL |
| Detector Antibody Diluent | 1 x 12 mL |
| Conjugate Diluent | 1 x 12 mL |
| 30X Wash Buffer | 1 x 20 mL |
| TMB Substrate | 1 x 9 mL |
| Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_955360 |
| Nucleotide Accession Num |
NM_199328 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBSW8 |
| Species Reactivity |
Human |
