CLMP ELISA Kit (Human) (GWB-KBBJH7) (Aviva Cat. No. OKDD00194)

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GWB-KBBJH7

Aviva Cat. No.: OKDD00194

Additional Information

Name CLMP ELISA Kit (Human) (GWB-KBBJH7)
Kit Range 0.156-10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type Serum, plasma, tissue homogenates
Kit Detection Absorbance, OD 450 nm
Gene Symbol CLMP
Gene id 79827
Alias Symbols ACAM, Adipocyte adhesion molecule, ASAM, CAR-like membrane protein, Coxsackie-and adenovirus receptor-like membrane protein, FLJ22415, UNQ318/PRO363
Swissprot ID Q9H6B4
Protein Name CXADR-like membrane protein
Description of Target This gene encodes a type I transmembrane protein that is localized to junctional complexes between endothelial and epithelial cells and may have a role in cell-cell adhesion. Expression of this gene in white adipose tissue is implicated in adipocyte matur
Kit Principle Genway Biotech CLMP ELISA Kit (Human) (GWB-KBBJH7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLMP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLMP captured in the well.
Kit Component
ComponentAmount
Anti-CLMP Microplate96 Wells (12 x 8 Well Strips)
CLMP Lyophilized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CLMP Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_079045.1
Nucleotide Accession Num NM_024769.3
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3.5 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBJH7
Species Reactivity Human