CLMP ELISA Kit (Human) (GWB-KBBUZ1) (Aviva Cat. No. OKCD00649)

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Please see item number OKCD00649 to view product details and for ordering information.


GWB-KBBUZ1

Aviva Cat. No.: OKCD00649

Additional Information

Name CLMP ELISA Kit (Human) (GWB-KBBUZ1)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CLMP
Gene id 79827
Alias Symbols ACAM, Adipocyte adhesion molecule, ASAM, CAR-like membrane protein, Coxsackie-and adenovirus receptor-like membrane protein, FLJ22415, UNQ318/PRO363
Swissprot ID Q9H6B4
Protein Name CXADR-like membrane protein
Description of Target May be involved in the cell-cell adhesion. May play a role in adipocyte differentiation and development of obesity. Is required for normal small intestine development
Kit Principle Genway Biotech CLMP ELISA Kit (Human) (GWB-KBBUZ1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLMP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLMP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLMP captured in the well.
Kit Component
ComponentAmount
Anti-CTBS Microplate96 Wells (12 x 8 Well Strips)
CTBS Lyophilized Standard2 x 10 ng
100X Biotinylated CTBS Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_079045
Nucleotide Accession Num NM_024769
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)86-102%88-96%85-98%99-105%
EDTA Plasma (n=5)85-99%80-90%93-102%92-105%
Heparin Plasma (n=5)88-98%90-97%84-102%95-102%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)89-9995
EDTA Plasma (n=5)78-9382
Heparin Plasma (n=5)98-105102
Datasheets/Manuals Printable datasheet for GWB-KBBUZ1
Species Reactivity Human