| Name |
CLOCK ELISA Kit (Human) (GWB-KBBSL6) |
| Kit Range |
0.312 - 20 ng/mL |
| Sensitivity |
< 0.117 ng/mL |
| Sample Type |
tissue homogenates, cell lysates and other biological fluids |
| Kit Detection |
Colorimetric, OD 450 nm |
| Gene Symbol |
CLOCK |
| Gene id |
9575 |
| Alias Symbols |
bHLHe8, BHLHE8, Circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, hCLOCK, KAT13D, KIAA0334 |
| Swissprot ID |
O15516 |
| Protein Name |
circadian locomoter output cycles protein kaput |
| Description of Target |
Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expres |
| Kit Principle |
Genway Biotech CLOCK ELISA Kit (Human) (GWB-KBBSL6) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CLOCK has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CLOCK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CLOCK captured in the well. |
| Kit Component |
| Component | Amount |
|---|
| Anti-Mtnd1 Microplate | 96 Wells (12 x 8 Well Strips) | | Mtnd1 Lyophilized Standard | 2 x 20 ng | | 100X Biotinylated Mtnd1 Detector Antibody | 120 uL | | Avidin/HRP Conjugate | 120 uL | | Standard Diluent | 1 x 20 mL | | Detector Antibody Diluent | 1 x 12 mL | | Conjugate Diluent | 1 x 12 mL | | 30X Wash Buffer | 1 x 20 mL | | TMB Substrate | 1 x 9 mL | | Stop Solution | 1 x 6 mL |
|
| Protein Accession Num |
NP_001276755 |
| Nucleotide Accession Num |
NM_001289826 |
| Assay Info |
Assay Methodology: Quantitative Sandwich ELISA |
| Reconstitution and Storage |
Store at 2 - 8C. Do not use the kit beyond the expiration date. |
| Kit Duration |
~ 3 Hours |
| Datasheets/Manuals |
Printable datasheet for GWB-KBBSL6 |
| Species Reactivity |
Human |
