Clock ELISA Kit (Mouse) (GWB-KBBKT4) (Aviva Cat. No. OKCD02092)

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Please see item number OKCD02092 to view product details and for ordering information.


GWB-KBBKT4

Aviva Cat. No.: OKCD02092

Additional Information

Name Clock ELISA Kit (Mouse) (GWB-KBBKT4)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.063 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CLOCK
Gene id 12753
Alias Symbols 5330400M04Rik, bHLHe8, Circadian locomoter output cycles protein kaput, KAT13D, mCLOCK, mKIAA0334
Swissprot ID O08785
Protein Name Circadian locomoter output cycles protein kaput
Description of Target Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expres
Kit Principle Genway Biotech Clock ELISA Kit (Mouse) (GWB-KBBKT4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Clock has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Clock is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Clock captured in the well.
Kit Component
ComponentAmount
Anti-Clock Microplate96 Wells (12 x 8 Well Strips)
Clock Lyophilized Standard2 x 10 ng
100X Biotinylated Clock Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001276755
Nucleotide Accession Num NM_001289826
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBKT4
Species Reactivity Mouse