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CNR1 ELISA Kit (Human) (GWB-KBBKS1)

GWB-KBBKS1

96 Wells
$605.00
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Additional Information

Name CNR1 ELISA Kit (Human) (GWB-KBBKS1)
Gene Symbol CNR1
Gene id 1268
Alias Symbols CANN6, Cannabinoid receptor 1, CB1, CB1A, CB1K5, CB1R, CB-R, CNR
Protein Name cannabinoid receptor 1
Description of Target This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protei
Swissprot ID P21554
Protein Accession Num NP_001153698.1
Nucleotide Accession Num NM_001160226.1
Species Reactivity Human
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Sensitivity < 0.046 ng/mL
Kit Range 0.156-10 ng/mL
Sample Type Serum, Plasma, Tissue homogenates, Cell lysates
Datasheets / Downloads GWB-KBBKS1 Datasheet
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Principle Genway Biotech CNR1 ELISA Kit (Human) (GWB-KBBKS1) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CNR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CNR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CNR1 captured in the well.
Kit Component
ComponentAmount
Anti-CNR1 Microplate96 Wells (12 x 8 Well Strips)
CNR1 Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X CNR1 Biotinylated Detector Antibody1 x 120 µL
100X Avidin-HRP Detection Conjugate1 x 120 µL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Kit Detection Absorbance, OD 450 nm
Kit Duration ~ 3.5 Hours