COL11A2 ELISA Kit (Human) (GWB-KBBIB8) (Aviva Cat. No. OKCD01636)

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Please see item number OKCD01636 to view product details and for ordering information.


GWB-KBBIB8

Aviva Cat. No.: OKCD01636

Additional Information

Name COL11A2 ELISA Kit (Human) (GWB-KBBIB8)
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.067 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol COL11A2
Gene id 1302
Alias Symbols Collagen alpha-2(XI) chain, DFNA13, DFNB53, HKE5, PARP, STL3
Swissprot ID P13942
Protein Name Collagen alpha-2(XI) chain
Description of Target May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Kit Principle Genway Biotech COL11A2 ELISA Kit (Human) (GWB-KBBIB8) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COL11A2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COL11A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample COL11A2 captured in the well.
Kit Component
ComponentAmount
Anti-COL11A2 Microplate96 Wells (12 x 8 Well Strips)
COL11A2 Lyophilized Standard2 x 10 ng
100X Biotinylated COL11A2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001157243
Nucleotide Accession Num NM_001163771
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)88-103%84-101%90-99%79-95%
EDTA Plasma (n=5)78-101%97-104%93-102%80-105%
Heparin Plasma (n=5)88-96%78-97%79-90%98-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9187
EDTA Plasma (n=5)97-104101
Heparin Plasma (n=5)82-10195
Datasheets/Manuals Printable datasheet for GWB-KBBIB8
Species Reactivity Human