The GenWay Biotech, Inc. Cortisol ELISA Kit is intended for the quantitative measurement of Cortisol in human serum orplasma.
SUMMARY AND EXPLANATION
Cortisol (hydrocortisone, compound F) is the most potent glucocorticoid synthesized from cholesterol. Cortisol is found inthe blood either as free Cortisol, or bound to corticosteroid‐binding globulin (CBG). Cortisol production has an ACTH‐dependent circadian rhythm with peak levels in the early morning and a nadir at night. The factors controlling this circadianrhythm are not completely defined. Serum levels are highest in the early morning and decrease throughout the day. In themetabolic aspect, Cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization.Immunologically, Cortisol functions as an important anti inflammatory, and plays a role in hypersensitivity,immunosuppression, and disease resistance. It has also been shown that plasma Cortisol levels elevate in response tostress. Abnormal Cortisol levels are seen with a variety of different conditions: with adrenal tumors, prostate cancer,depression, and schizophrenia. Elevated Cortisol levels and lack of diurnal variation have been identified in patients withCushing's disease
PRINCIPLE OF THE TEST
The GenWay Cortisol ELISA is a solid phase competitive ELISA. The samples and Cortisol enzyme conjugate are added to thewells coated with anti‐Cortisol monoclonal antibody. Cortisol in the patient’s sample competes with a Cortisol enzymeconjugate for binding sites. Unbound Cortisol and Cortisol enzyme conjugate is washed off by washing buffer. Upon theaddition of the substrate, the intensity of color is inversely proportional to the concentration of Cortisol in the samples. Astandard curve is prepared relating color intensity to the concentration of the Cortisol