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Additional Information

Name CPA3 ELISA Kit (Human) (GWB-KBBFX9)
Description of Target This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein is proteolytically processed to generate a mature protease that is released by mast cells and may be involved in the degradation of endogenous
Kit Range 7.8 - 500 pg/mL
Sensitivity < 3.0 pg/mL
Sample Type serum, plasma and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CPA3
Gene id 1359
Alias Symbols Carboxypeptidase A3, Mast cell carboxypeptidase A, MC-CPA
Swissprot ID P15088
Protein Name Mast cell carboxypeptidase A
Kit Principle Genway Biotech CPA3 ELISA Kit (Human) (GWB-KBBFX9) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPA3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPA3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPA3 captured in the well.
Kit Component
Anti-CPA3 Microplate96 Wells (12 x 8 Well Strips)
CPA3 Lyophilized Standard2 x 500 pg
100X Biotinylated CPA3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001861
Nucleotide Accession Num NM_001870
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-99%93-101%99-105%86-94%
EDTA Plasma (n=5)84-99%78-94%92-99%91-101%
Heparin Plasma (n=5)80-102%79-98%79-90%85-101%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9996
EDTA Plasma (n=5)99-105102
Heparin Plasma (n=5)94-10298
Datasheets/Manuals Printable datasheet for GWB-KBBFX9
Species Reactivity Human