CPN1 ELISA Kit (Human) (GWB-KBBPJ4)

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GWB-KBBPJ4

96 Wells
$659.00
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Additional Information

Name CPN1 ELISA Kit (Human) (GWB-KBBPJ4)
Kit Range 15.62 - 1,000 pg/mL
Sensitivity < 6.2 pg/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CPN1
Gene id 1369
Alias Symbols ACBP, Anaphylatoxin inactivator, Arginine carboxypeptidase, Carboxypeptidase N catalytic chain, Carboxypeptidase N polypeptide 1, Carboxypeptidase N small subunit, CPN, FLJ40792, Kininase-1, Lysine carboxypeptidase, Plasma carboxypeptidase B, SCPN, Serum
Swissprot ID P15169
Protein Name carboxypeptidase N catalytic chain
Description of Target Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and an
Kit Principle Genway Biotech CPN1 ELISA Kit (Human) (GWB-KBBPJ4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPN1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPN1 captured in the well.
Kit Component
ComponentAmount
Anti-CPN1 Microplate96 Wells (12 x 8 Well Strips)
CPN1 Lyophilized Standard2 x 1 ng
100X Biotinylated CPN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001299
Nucleotide Accession Num NM_001308
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)93-101%88-105%95-104%78-104%
EDTA Plasma (n=5)89-103%82-94%87-94%79-104%
Heparin Plasma (n=5)89-97%87-103%92-101%89-96%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)80-9184
EDTA Plasma (n=5)78-10486
Heparin Plasma (n=5)80-10389
Datasheets/Manuals Printable datasheet for GWB-KBBPJ4
Species Reactivity Human