CPN2 ELISA Kit (Human) (GWB-KBBXU4) (Aviva Cat. No. OKCD00699)

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Please see item number OKCD00699 to view product details and for ordering information.


Aviva Cat. No.: OKCD00699

Additional Information

Name CPN2 ELISA Kit (Human) (GWB-KBBXU4)
Kit Range 1.56 - 100 ng/mL
Sensitivity < 0.63 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol CPN2
Gene id 1370
Alias Symbols ACBP, Carboxypeptidase N 83 kDa chain, Carboxypeptidase N large subunit, Carboxypeptidase N polypeptide 2, Carboxypeptidase N regulatory subunit, Carboxypeptidase N subunit 2
Swissprot ID P22792
Protein Name Carboxypeptidase N subunit 2
Description of Target The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
Kit Principle Genway Biotech CPN2 ELISA Kit (Human) (GWB-KBBXU4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CPN2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CPN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CPN2 captured in the well.
Kit Component
Anti-EFEMP1 Microplate96 Wells (12 x 8 Well Strips)
EFEMP1 Lyophilized Standard2 x 100 ng
100X Biotinylated EFEMP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001073982
Nucleotide Accession Num NM_001080513
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Kit linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)97-104%84-102%80-90%96-104%
EDTA Plasma (n=5)88-96%87-94%86-97%80-96%
Heparin Plasma (n=5)81-88%90-98%78-93%95-105%
Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)78-9490
EDTA Plasma (n=5)78-9890
Heparin Plasma (n=5)95-10399
Datasheets/Manuals Printable datasheet for GWB-KBBXU4
Species Reactivity Human